You can download the most recent version of FlowJo from: This tutorial is designed to walk you through the data analysis process in FlowJo from adding data to producing tables of statistics and publication-quality graphics. Therefore, it is possible that the graphics shown in this tutorial may not exactly match the windows that you see when you run the most recent version of FlowJo.
As a note, we are pleased to be able to frequently update FlowJo to provide new features and analysis capabilities. In addition, contains a page for FlowJo FAQs, tutorial videos, and the Daily Dongle, a blog discussing all things FlowJo. From this website, you can navigate the help pages to learn more about FlowJo. Pressing the question mark button in any window in FlowJo will launch a web browser and and access a help pages relevant to that window. You can learn more about FlowJo through our online help documents. and tools to export raw gated data for analysis in other programs, etc.). FlowJo is capable of much more that simply can t be covered in a tutorial like this (for example, there are analysis platforms for DNA/Cell Cycle analysis, Kinetics, Proliferation, etc. As you watch FlowJo perform various operations such as creating new graphs, statistics, tables, or graphical layouts, you will see how fast and easy FlowJo is to use. Run the program as you perform the steps in the tutorial, so that you can get the best feel for how the program works.
Reading through it, you will learn how to operate FlowJo. This tutorial is designed to cover a majority of the features pf the program. The Workspace is saved as a FlowJo document on your hard disk when you reopen the document, you will see your analysis as it was when it was last saved.
The environment is presented as the Workspace which contains a list of all of loaded samples (experimental data), statistics, gates, other analyses, as well as tabular and graphical layouts.
8 Color PBMC MMXII Revision Date: 14 May, 2013 Version iģ Introduction FlowJo is a software application with an integrated environment for viewing and analyzing flow cytometric data. Flowjo, its tutorials, documentation and website are copyright Tree Star, Inc All rights reserved. We are indebted to our active and enthusiastic users worldwide for their ideas, discussions and tireless testing of new versions. 2 Flowjo was written by Adam Treister and Mario Roederer beginning in 1996, based on concepts developed at the Herzenberg Laboratory at Stanford.